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Optical Microscope; • Scanning Electron Microscope (SEM ...

Lecture 3 Brief Overview of Traditional Microscopes Optical Microscope ; Scanning Electron Microscope (SEM); Transmission Electron Microscope (TEM); Comparison with Scanning probe Microscope (SPM) General philosophy Human beings use two kinds of means to gauge objectives: Seeing --- through eyes (light) Optical Microscope electronic Microscope (higher resolution due to short wavelength); Touching --- through hands (probe) SPM. Converting wavelength (nm) to Electron energy (eV): (nm), for example, a 500 nm light corresponds to = eV Principles of an Optical Microscope Development of Microscope takes us back to 400 years ago Robert Hooke s "Micrographia" (1665) A hair knot Room dusts Red blood cell Flu virus Organic nanobelts with strong emission Advantages and Disadvantages of Optical Microscope Advantages: Direct imaging with no need of sample pre-treatment, the only microscopy for real color imaging.

prototype electron microscope in 1931, capable of four-hundred-power magnification; the apparatus was the first demonstration of the principles of electron microscopy. Two years later, in 1933, Ruska built an electron microscope that exceeded the resolution attainable with an optical (light) microscope.

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  Optical, Electron, Scanning, Microscope, Optical microscope scanning electron microscope

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Transcription of Optical Microscope; • Scanning Electron Microscope (SEM ...

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