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Optical Microscope; • Scanning Electron Microscope (SEM ...

Lecture 3 Brief Overview of Traditional Microscopes Optical Microscope ; Scanning Electron Microscope (SEM); Transmission Electron Microscope (TEM); Comparison with Scanning probe Microscope (SPM) General philosophy Human beings use two kinds of means to gauge objectives: Seeing --- through eyes (light) Optical Microscope electronic Microscope (higher resolution due to short wavelength); Touching --- through hands (probe) SPM. Converting wavelength (nm) to Electron energy (eV): (nm), for example, a 500 nm light corresponds to = eV Principles of an Optical Microscope Development of Microscope takes us back to 400 years ago Robert Hooke s "Micrographia" (1665) A hair knot Room dusts Red blood cell Flu virus organic nanobelts with strong emission Advantages and Disadvantages of Optical Microscope Advantages: Direct imaging with no need of sample pre-treatment, the only microscopy for real color imaging.

a small probe and is rastered across the surface of a specimen. ... • Sample and sample holder: 1. size --- centimeters. 2. rotation --- sample can be rotated freely at three dimensions, x, y, z, to achieve imaging ... of organic materials, • Electron diffraction technique:

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Lecture 10: Basics of Atomic Force Microscope (AFM)

my.eng.utah.edu

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