Transcription of OptiPrep™ Preparation of gradient solutions
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OptiPrep™ Preparation of gradient solutions
optiprep Preparation of gradient solutions Measurement and calculation of densities The density of optiprep solutions can be determined directly and accurately by weighing a known volume using a density bottle. Weighing a series of aliquots dispensed from an automatic pipette (calibrated with water) on an electronic balance is a permissible but less accurate alternative. However the most convenient way of determining the density is to measure the refractive index. Another method for determining the density of a solution is to measure its absorbance. If a solution of iodixanol of known density is mixed with a diluent (whose density is also known), the density of the mix-ture can be calculated from the following equation: D = Density of mixture; V = volume of iodixanol solu-tion; d = density of iodixanol solution; V1 = volume of diluent; d1 = density of diluent. optiprep working and gradient solutions for mammalian cells An illustrative example for preparing working solutions for cells, based on a cell suspension medium (CSM) con-taining (w/v) NaCl, 10 mM Tricine-NaOH, pH , is given in the Table 1 below.
OptiPrep™ Preparation of gradient solutions Measurement and calculation of densities tion would also contain 3 mM CaCl The density of OptiPrep™ solutions can be ...
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