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Oxidase Test Protocol - American Society for Microbiology

Downloaded from byIP: : Mon, 12 Aug 2019 20:11:08 American Society for Microbiology 2016 1 Oxidase Test Protocol | | Created: Thursday, 11 November 2010 Author Patricia Shields Laura Cathcart Information History In 1928, Gordon and McLeod (5) introduced the use of a dimethyl-p-phenylenediamine dihydrochloride solution to test for the presence of Oxidase systems. In particular, they used the test to distinguish Neisseria gonorrhoeae( Oxidase positive) from Staphylococcus spp. and Streptococcus spp. ( Oxidase negative). The sensitivity of the Oxidase test was increased when Kov cs found that a tetra-methyl-p-phenylenediamine dihydrochloride solution gave a quicker reaction (8).

Recommendations: Introductory Course in Microbiology and the Guidelines for Biosafety in Teaching Laboratories. COMMENTS AND TIPS The reagents used in the oxidase test have been shown to autooxidize, so it is very important to use fresh reagents, no older than 1 week (2, 3, 14, 15). Steel found that the autooxidation can be

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