Transcription of P rotein preparation handbook - Thermo Fisher Scientific
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P rotein preparation handbook - Thermo Fisher Scientific
Protein preparation handbookCell lysis | Subcellular fractionation | Protease and phosphatase inhibition | Dialysis | Desalting | Concentration | Purification | Immunoprecipitation | Co-immunoprecipitation | Pull-down2We offer a full range of optimized tools for efficient protein extraction, fractionation and targeted inhibition of unwanted protease and phosphatase activity. Our convenient devices and high-performance affinity resins and magnetic beads enable maximum yield for the purification, enrichment, and cleanup of proteins and antibodies for downstream extractionProtein extraction techniques vary depending on the source of the starting material, the location of the protein of interest within the cell, and the downstream application.
Cell lysis disrupts cell membranes and organelles, resulting in unregulated enzymatic activity that can reduce protein yield and function. To prevent these negative effects, protease and phosphatase inhibitors ... exchange, the retentate is diluted to the original volume
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