Transcription of PerfeCT MultiPlex qPCR ToughMix - quantabio.com
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PerfeCT MultiPlex qPCR ToughMix - quantabio.com
95147 / REV 02 1 PerfeCTa MultiPlex qPCR ToughMix Description PerfeCTa MultiPlex qPCR ToughMix is a 5X concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta MultiPlex qPCR ToughMix has been optimized to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.
95147 / IFU-101.1 REV 02 2 Guidelines for qPCR continued: Suggested input quantities of template are: cDNA corresponding to 10 pg to 1 µg of …
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