Peripheral Blood Mononuclear Cell (PBMC) Isolation and Red …
2. Mix 1 ml of blood with 1 ml of a balanced salt solution, normally PBS. 3. Overlay the mixed blood solution on top of the Ficoll-Paque PLUS or Histopaque-1077. Ratio should be 2 ml of the mixed blood + PBS solution for every 1 ml of Ficoll-Paque PLUS or Histopaque-1077. 4. Centrifuge at 400 x g for 35 minutes at room temperature.
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