Transcription of pET System Manual - BGU
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pET System Manual - BGU
PET System Manual TB055 10th Edition 0403 1 United States & Canada 800-207-0144 Germany 0800 6931 000 United Kingdom 0800 622935 Or your local sales office Novagen This second printing of the 10 th edition of the pET Manual was published May, 2003. Novagen is continually expanding and upgrading the pET System . Please check the Novagen website, , for updated pET System Manual information. Table of Contents Table of Contents 1 I. About the System 4 A. Description 4 B. Licensing and Use Agreement 4 C. System Components 4 D. Choosing a pET Vector 5 Primary considerations 5 Solubility and cellular localization 6 Fusion tags 6 pET vector characteristics table 8 E. pET Vector Cloning Strategies 9 Produce native proteins without fusions 9 Produce native proteins without fusions after protease cleavage 10 Ligation-independent cloning 10 F. Regulating Protein Expression in the pET System 11 The T7lac promoter 11 pLysS and pLysE hosts 11 Vector and host combinations affect expression levels 12 Media containing glucose 12 pLacI hosts 13 pETcoco System 13 G.
pET System Manual 2 TB055 10th Edition Rev. B 0403 United States & Canada 800-207-0144 Germany 0800 6931 000 United Kingdom 0800 622935 Or your local sales office www.novagen.com
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