Transcription of Pretransfusion Testing (Basic Immunohematology …
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Pretransfusion Testing (Basic Immunohematology …
Pretransfusion Testing (Basic Immunohematology Part 2). February 2012. D. Joe Chaffin, MD. I. Outline: A. Overview of Pretransfusion Testing B. Testing methods C. Required components of the process D. Nomenclature of tests used II. Overview A. Purpose 1. Provide a blood component to a patient that will provide maximum benefit while minimizing potential for harm a) Choose the most appropriate blood product from a safe, compatible donor and infuse it into a properly identified and thoroughly tested recipient 2. This requires a systematic process that is fairly rigid 3. Regulated in the US by: a) CLIA (Clinical Laboratory Improvement Amendments, 1988). (1) Determines the minimum standards for lab operations; inspections regulated by Centers for Medicare and Medicaid Services (CMS).
(4) pH (a) Optimal pH (7.0 in vitro) encourages an environment where: i) RBC surfaces are negatively charged ii) Antibodies are weakly positive (b) Decreasing pH leads to dissociation of antibody from RBC surface
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