引物设计和Primer-BLAST的应用

Primer-BLAST 2. 3. 4. polymerase chain Reaction 1971 Khorana 1985 KaryMullis PCR1986 5 Mullis The Cold Spring Harbor Laboratory CSHL PCR 1. DNA PCR PCR Inverse PCR IPCR PCR anchored PCR RACE Rapid Amplification of cDNA Ends PCR ligation-mediated PCR LM-PCR 2. PCR nested PCR 3. PCR PCR real-time quantitative PCR RQ-PCR G+C 40%-60% 4 18-27 3bp 3bp 3 Tm Tm 5 C Tm 10 C3 3 G C 3 NNGC NNCG GC rich AT rich 3 T/C A/G Primer Premier 6 P6 Beacon Designer 8 DB Oligo 6 Vector NTI Suit Primer Express PCR *Omiga* serpine1 NCBI Reference Sequence.

Madden1，Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction, Ye et al. BMC Bioinformatics 2012, 13:134 4.刘驰, 李家宝, 芮俊鹏, 安家兴, 李香真.16S rRNA 基因在微生物生态学中的应用.生态学报, 2015, 35(9):2769-2788

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  Chain, Reactions, Polymerase chain reaction, Polymerase

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