Transcription of Probe Design, Production, and Applications
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Probe Design, Production, and Applications
Probe design , production , Applications1313 From: Medical Biomethods HandbookEdited by: J. M. Walker and R. Rapley Humana Press, Inc., Totowa, NJ2 Probe design , production , and ApplicationsMarilena Aquino de Muro1. IntroductionA Probe is a nucleic acid molecule (single-stranded DNA or RNA) with a strong affinitywith a specific target (DNA or RNA sequence). Probe and target base sequences must becomplementary to each other, but depending on conditions, they do not necessarily have to beexactly complementary. The hybrid ( Probe target combination) can be revealed when appro-priate labeling and detection systems are used. Gene probes are used in various blotting and insitu techniques for the detection of nucleic acid sequences. In medicine, they can help in theidentification of microorganisms and the diagnosis of infectious, inherited, and other Probe DesignThe Probe design depends on whether a gene Probe or an oligonucleotide Probe is Gene ProbesGene probes are generally longer than 500 bases and comprise all or most of a target can be generated in two ways.
14 Aquino de Muro its target sequence and is sufficiently long to allow the use of hybridization conditions that will prevent the hybridization to other closely related sequences, making it possible to identify and
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