Transcription of Protein-liposome conjugates with defined size distributions
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Protein-liposome conjugates with defined size distributions
Biochimica et Biophysica Acta, 1028(1990) 73-81 73. Elsevier BBAMEM74971. Protein-liposome conjugates with defined size distributions H . C . L o u g h r e y 1, K . F . W o n g 1, C h o i 2, P . R . Cullis 1,2 a n d M . B . B a l l y 2. 1 The Unioersity of British Columbia, Faculty of Medicine, Department of Biochemistry, Vancouver, and 2 The Canadian liposome Co. Ltd., North Vancouver, (Canada). (Received2 January 1990). (Revisedmanuscript received15 May 1990). Key words: protein -liposomeconjugate; Streptavidin; Biotin; Vesicleaggregation; Liposomepreparation; Freeze-fracture Conjugation of protein to liposomes by two coupling protocols is shown to result in vesicle aggregation. The degree of aggregation is directly related to the levels of protein conjugated to the liposomes. In an attempt to develop a method of generating stable, homogeneously sized protein -conjugated vesicles, highly aggregated liposome - protein conjugates were extruded through filters of defined pore size .
cubated with [3H]- or [14C]biotin (3.85 nmol in 25 #1, 15.4 nmol/gCi) for 10 rain and unbound biotin was removed by gel filtration on Sepharose CL-4B equi-
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