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2009 Life Technologies CorporationProtein Thermal Shift Assay Using Applied Biosystems real Time PCR SystemsMadeline O Donoghue1, Patrice Allard1, Astrid Ferlinz1, Joris Verheyde1, Kelly Warrington1 Naima Nhiri2, Eric Jacquet21 Applied Biosystems , part of Life Technologies, Foster City, CA. 2 IMAGIF, B t 23B, ICSN-CNRS, Gif-sur-Yvette, France, 6. Normalized Melt Curve data generated on a 7900 HT real Time PCR System showing the effect of a specific ligand on Protein stability. The Tm of the curve increases over 10oC in the presence of the 5. Derivative Melt Curve data from a 7900 HT real Time PCR System depicting a TSA Assay measuring the effect of salt concentrations on Protein thermostability.
© 2009 Life Technologies Corporation Protein Thermal Shift Assay Using Applied Biosystems Real Time PCR Systems. Madeline O’Donoghue. 1, Patrice Allard
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