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PURE CULTURE TECHNIQUES

pure CULTURE TECHNIQUES . Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example, has over 1010 bacteria and that gram would have over 20 different bacterial species. Particularly in a medical setting, where a patient's body fluid or tissue (skin, blood, spinal fluid, urine) is sent to the microbiology lab for analysis, the specimens will most often be mixed. In order to identify the bacteria and run antibiotic susceptibilities on the causative agent of the infection, the microbiologist must be working with a pure CULTURE of the bacterium. This exercise begins with a mixed CULTURE of bacteria and will end with, hopefully, pure cultures of the 2 bacteria. Luckily, the 2 bacteria being used look different from each other when growing on agar plates. Two different types of agar plate isolation TECHNIQUES will be used---streak and pour.

PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (o r other microorganisms).

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  Culture, Pure, Pure culture

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