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Q Exactive Mass Spectrometer Series Calibration and ...

The world leader in serving science Tatjana Talamantes and Roberto Gamez North America Omics Markets Field Support, Center of Excellence HRAM iQuan 2016: Thermo Scientific Q Exactive Mass Spectrometer Series Calibration & Maintenance 2 HRAM iQuan Agenda Thermo Scientific Q Exactive Mass Spectrometer Calibration & Maintenance Tuning and Calibration Tune Software Interface ( ) Preparation Tuning for Calibration Solvents Calibration Procedure Maintenance Considerations Schedules How-to s 3 Calibration Preparation, Tuning, Calibration 4 Q Exactive MS Series Tune Interface (Tune ): Overview User Role Display Panel Menu Bar Toolbar Tasks Panel 5 Q Exactive MS Series Tune Interface (Tune ): Display Panel Options Instrument Messages Displayed here and saved in log files Alerts the user if the system noticed a problem 6 Q Exactive MS Series Tune Interface (Tune ): Display Panel Options Analysis Graphs When instrument plots something, the graphs will display here Graphs can be copied to the clipboard Graphs are not automatically saved 7 Q Exactive MS Series Tune Interface (Tune ): Display Panel Options Tune available now!

Sheath gas flow is typically set low, Aux gas flow is either not required or set lower than Sheath . Spray voltage for positive and negative mode is between 3 and 4.5 kV Capillary temp (set at 320 °C for calibration) S-lens level may have to be adjusted manually (typical range is 50-60) HESI-II Probe Heater temp is NOT used and can be set to 0 °C

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