QuantiLyse: Reliable DNA Amplification from Single …

QuantiLyse : Reliable DNA Amplification from Single cells Kenneth E. Pierce, John E. Rice, J. Aquiles Sanchez, and Lawrence J. Wangh Brandeis University, Waltham, MA, USA. BioTechniques 32:1106-1111 (May 2002). ABSTRACT of PCR-based assays in both scientific investigations and clin- ical diagnosis. Although the introduction of real-time PCR in Amplification of DNA sequences from Single cells via PCR is in- the last few years has improved the quantitative rigor of such creasingly used in basic research and clinical diagnostics but re- assays, their reliability at the Single -cell level remains prob- mains technically difficult. We have developed a cell lysis protocol lematic.

ABSTRACT Amplification of DNA sequences from single cells via PCR is i-n creasingly used in basic research and clinical diagnostics but r- e mains technically difficult.

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