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Quantitative Analysis of Underivatized Amino Acids …

Application NoteFood Testing, Metabolomics, Agricultural Chemistry, EnvironmentalAuthorsYuxiong Huang, Weiwei Li, and Arturo Keller University of California, Santa BarbaraTarun Anumol and Adam Bivens Agilent Technologies, Application Note presents a method for Quantitative Analysis of a wide range of Underivatized Amino Acids by triple quadrupole LC/MS. Sensitivity, linearity, and recovery are shown to be excellent, even in the presence of significant amounts of plant matrix (cucumber plant tissue extract). Hydrophilic interaction chromatography (HILIC) was used to separate the highly polar Amino Acids . The next generation Agilent InfinityLab Poroshell 120 HILIC-Z phase was used to take advantage of its excellent resolution and peak shape under LC/MS friendly Analysis of Underivatized Amino Acids in Plant Matrix by Hydrophilic Interaction Chromatography (HILIC) with LC/MS Detection2 IntroductionA wide range of Amino Acids are present in plant material at parts-per-million (ppm) and parts-per-billion (ppb) levels.

2 Introduction A wide range of amino acids are present in plant material at parts-per-million (ppm) and parts-per-billion (ppb) levels. As previously found1, underivatized amino acids can be analyzed with excellent resolution and sensitivity using

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