Transcription of QUICK REFERENCE Qubit Assays - Thermo Fisher Scientific
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QUICK REFERENCE Qubit Assays - Thermo Fisher Scientific
QUICK REFERENCEQUICK REFERENCEQ ubit AssaysFor Research Use Only. Not for use in diagnostic procedures. Pub. No. MAN 0 017210 Rev. REFERENCE Qubit Assays1. Set up two assay tubes for the standards (three forthe protein or RNA IQ assay ) and one assay tube foreach Prepare the Qubit working solution by diluting theQubit reagent 1:200 in Qubit buffer. Prepare 200 L ofworking solution for each standard and sample. 3. Prepare the assay tubes* according to the table assay tubesUser sample assay tubesWorking solution (from step 2)190 L180 199 LStandard (from kit)10 L User sample 1 20 LTotal Volume in each assay tube200 L200 L Qubit 1X dsDNA Assays (Cat. Nos. Q33230, Q33231, Q33265, Q33266) are supplied with a ready-to-use working solution, and do not require preparation. * Use only thin-wall, clear mL PCR tubes. Acceptable tubes include Qubit assay tubes (set of 500, Cat. No. Q32856).General Qubit assay Protocol4. Vortex all tubes for 2 3 Incubate the tubes for 2 minutes at room temperature(15 minutes for the Qubit protein assay ).
QUICK REFERENCE . Qubit ™ Assays. 1. Set up two assay tubes for the standards (three for. the protein or RNA IQ assay) and one assay tube for each sample. 2. Prepare the Qubit ™ working solution by diluting the Qubit ™ reagent 1:200 in Qubit buffer. Prepare 200 μL of working solution for each standard and sample. † 3.
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