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Real-Time PCR Applications Guide - Bio-Rad Laboratories

Applications GuideReal-Time PCR Applications Guide table of contentstable of contents iTable of Contents1. Overview of Real-Time Key Concepts of Real-Time What Is Real-Time PCR? How Real-Time PCR Hallmarks of an Optimized qPCR Assay42. Getting General Experimental Design Considerations for Singleplex or Multiplex? Chemistry DNA-Binding Dyes (SYBR Green I) Fluorescent Primer- and Probe-Based Design and Optimization of SYBR GreenI Primer and Amplicon Assay Validation and Annealing Temperature Assay Performance Evaluation Using Standard Design and Optimization of TaqMan Probe Primer and Probe Assay Validation and Optimization243.

7.2 DNA Extraction and Sample Preparation for GMO Detection 77 7.3 GM Soy Detection Using a Singleplex SYBR Green I qPCR Assay 78 7.3.1 Reaction Components 78 7.3.2 Cycling Protocol 79 7.3.3 Data Analysis 79 7.4 GM Soy Detection Using a Multiplex TaqMan qPCR Assay 81 7.4.1 Reaction Components 82 7.4.2 Cycling Protocol 82 7.4.3 Data Analysis 82 8.

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  Laboratories, Extraction, Bio rad laboratories, Dna extraction

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