Real-time PCR handbook - Faculdade de Filosofia, Ciências ...

Real-time PCR handbookSingle-tube assays96- and 384-well plates384-well TaqMan Array CardsOpenArray platesCommonly used formats for Real-time Basics of Real-time PCR Digital PCR Experimental design Plate preparation Data analysis TroubleshootingBasics of Real-time Introduction Overview of Real-time PCR Overview of Real-time 4 PCR Real-time PCR analysis technology Real-time PCR fluorescence 10 detection Melting curve analysis Passive reference dyes Contamination prevention Multiplex Real-time PCR Internal controls and 18 reference Real-time PCR 19 instrument calibration1 Basics of Real-time PCR IntroductionThe polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology.

DNA polymerase, so enzyme selection is critical to success. One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA during reaction setup, allowing the polymerase to synthesize nonspecific product. The problem of

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