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Reverse-Phase High-Performance Liquid Chromatography ...

UC Berkeley College of ChemistryChemistry 105 Instrumental Methods in Analytical ChemistryReverse-Phase High-PerformanceLiquid Chromatography analysis ofBiomoleculesShort ReportAuthor:JonathanMelvilleLab Partners:JakePrecht, PhuongTran, and JeremyHsuGraduate Student Instructors:RichardCooper& DanielMortensenApril 23, 20141 TheoryRP-HPLC, or Reverse-Phase High-Performance Liquid Chromatography is a type of chro-matography the features a Liquid mobile phase, higher resolving power than traditionalliquid Chromatography , and a specific combination of stationary- and mobile-phases thatresults in separations that are the opposite of a traditional HPLC. Chromatogra-phy, in general, utilizes the flow of a mobile-phase containing the analyte througha stationary-phase depending on the intermolecular forces of attraction between theindividual analytes and the stationary- and mobile-phases, the various components ofthe sample will exit the chromatographic column at different times[?]

Liquid Chromatography Analysis of ... Jonathan Melville Lab Partners: Jake Precht, Phuong Tran, and Jeremy Hsu Graduate Student Instructors: Richard Cooper & Daniel Mortensen April 23, 2014. ... 2 A 1.518 2.129 0.193 0.201 3.1015228426 3 A 1.448 2.127 0.21 0.171 3.5643044619 A.1.6 0.1 ppm std. Trial Method Peak 1 (min) Peak 2 (min) Peak 1 width ...

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