Transcription of Reverse-Phase High-Performance Liquid Chromatography ...
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UC Berkeley College of ChemistryChemistry 105 Instrumental Methods in Analytical ChemistryReverse-Phase High-PerformanceLiquid Chromatography Analysis ofBiomoleculesShort ReportAuthor:JonathanMelvilleLab Partners:JakePrecht, PhuongTran, and JeremyHsuGraduate Student Instructors:RichardCooper& DanielMortensenApril 23, 20141 TheoryRP-HPLC, or Reverse-Phase High-Performance Liquid Chromatography is a type of chro-matography the features a Liquid mobile phase, higher resolving power than traditionalliquid Chromatography , and a specific combination of stationary- and mobile-phases thatresults in separations that are the opposite of a traditional HPLC.
the sample will exit the chromatographic \column" at di erent times[?]. A substance that experiences greater intermolecular forces of attraction to the stationary-phase will be slowed, and will exit the column later than a substance without such attraction. If the mobile-phase is a liquid (as opposed to, say, a gas), the process is considered ...
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