Transcription of Reversed Phase Chromatography - Wolfson Centre …
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Reversed Phase Chromatography - Wolfson Centre …
Reversed PhaseChromatographyPrinciples and Methods18-1134-16 Edition AAReversed PhaseChromatographyContents1. Introduction .. 5 Theory of Reversed Phase Chromatography .. 6 The matrix .. 9 The ligands .. 11 Resolution in Reversed Phase Chromatography .. 13 Resolution .. 13 Capacity factor .. 14 Efficiency .. 15 Selectivity .. 17 Binding capacity .. 18 Critical parameters in Reversed Phase Chromatography .. 19 Column length .. 19 Flow rate .. 19 Temperature .. 20 Mobile Phase .. 20 Organic solvent .. 20 Ion suppression .. 21 Ion pairing agents .. 22 Gradient elution .. 23 Mode of use .. 24 Desalting .. 24 High resolution separations .. 25 Large scale preparative purification .. 25 Stages in a purification scheme.
7 Fig. 2. Principle of reversed phase chromatography with gradient elution. Reversed phase chromatography of biomolecules generally uses gradient elution
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