Transcription of RNA Silencing, mechanism and applications
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RNA Silencing, mechanism and applications
RNA Silencing, mechanism and applicationsThe Central Dogma of Molecular BiologyThere is life outside protein-coding genes There are approximately gene predictions for the Human the estimated number of protein coding genes is 21,561 It is estimated that 98% of the transcriptional output of the human genome represents RNA that does not encode proteinRNA Silencing, mechanism and applications2. The role of small RNAs in gene regulation3. Mechanisms of RNA interference4. Biological role of RNA silencing5. applications of RNA silencing6. miRNAs1. History and definitionsPosttranscriptional gene silencing (PTGS)Posttranscriptional gene silencing (PTGS)Classical key experiment in petunia: Artificially introduced 2 nd CHS gene Expected: more intense flowersReal outcome: Variable phenotypes, including white flowers Key enzyme for flower pigmentation:chalcone synthase (CHS)van der Krolet al., Plant Cell 2:291 (1990) + Plant Mol Biol14:457 (1990).
Key experiment on RNA interference (RNAi) Nature 391, 806 - 811 (1998);Nature 391, 806 - 811 (1998); Injection of ds RNA to C. elegans causes gene-specific silencing Injection of ds RNA to C. elegans causes gene-specific silencing New term: RNA interference (RNAi) New term: RNA interference (RNAi)
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