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RSC Advances - gaomingyuan.com
Detection of Epstein Barr virus infection in cancerby using highly specific nanoprobe based on dBSAcapped CdTe quantum dots Yilin Li,abLihong Jing,bKe Ding,bJing Gao,aZhi Peng,aYanyan Li,aLin Shen*aand Mingyuan Gao*bBy using denatured bovine serum albumin (dBSA) molecules as multidentate ligands, surface-functionalizedCdTe quantum dots (QDs) were synthesized and further evaluated for preparingfluorescent probes capableof detecting Epstein Barr virus infection. The optical studies revealed that the dBSA coating effectivelyimproved the optical stability of the resulting QDs The interaction between CdTe@dBSAand the membrane of carcinoma cells further suggested that the nonspecific adsorption of CdTe QDsstabilized by thioglycolic acid (TGA) was significantly eliminated upon the dBSA coating. Streptavidin wascovalently conjugated to CdTe@dBSA to endow the QDs with specific binding affinity in detecting theserum level of anti-Epstein Barr virus (EBV) capsid antigen IgA (VCA-IgA) in nasopharyngeal carcinomapatients for early screening and diagnosing EBV-associated IntroductionQuantum dots (QDs) have been attracting considerable atten-tion owing to their unique electronic and optical 3As the spectral features of QDs in comparison with that ofconventional dyes is characterized by narrow, symmetric, andparticle size-dependent uorescence, as well as a broad excita-tion range and excellent
3. Results and discussion 3.1. dBSA-coated CdTe QDs The absorption and uorescence spectra of the as-prepared CdTeQDs stabilized byTGA, denotedas CdTe@TGA, are …
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