Transcription of Section 7 Basic principles of cryopreservation
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Section 7 Basic principles of cryopreservation
Section 7 Basic principles of cryopreservation85 Basic principles of cryopreservationSpermatozoa were the first mammalian cells to be cryopreserved successfully (Polge et al., 1949). This success was due to the serendipitous discovery by Polge and co-workers of the cryoprotective effect of glycerol. Since then, many methods have been developed for various types of cells, tissues and organs. Much progress in the field has come from empirical work as well as from fundamental cryobiology. Increased understanding of the causes of cryo-injury has continually helped to improve cryopreservation methods.
Basic principles of cryopreservation 89 and their membrane surface area (volume to surface area ratio), and by the permeability of the membrane to water and to cryoprotectant (see Figure 9).
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