Transcription of Selecting Buffer pH in Reversed-Phase HPLC
{{id}} {{{paragraph}}}
Example: tourism industry
Selecting Buffer pH in Reversed-Phase HPLC
8 Tel: 1-800-255-8324 Fax: 847-948-1078 Specialists in Chromatography Selecting the proper Buffer pH isnecessary to reproducibly separateionizable compounds by reversed-phaseliquid chromatography. Selecting animproper pH for ionizable analytes oftenleads to asymmetric peaks that are broad,tail, split, or shoulder. Sharp, symmetricalpeaks are necessary in quantitativeanalysis in order to achieve low detectionlimits, low relative standard deviations(RSD) between injections, andreproducible retention times. We willdiscuss when buffering is needed, how toselect Buffer pH, and how to choosethe proper Buffer system for that is Buffering Needed?
8 “Specialists in Chromatography” Tel: 1-800-255-8324 • Fax: 847-948-1078 Selecting the proper buffer pH is necessary to reproducibly separate ionizable compounds by reversed-phase liquid chromatography. Selecting an
Loading..
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document: