Transcription of Separation by Chromatography Methods - Sinica
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Separation by Chromatography Methods - Sinica
Separation by Chromatography Methods Analytical Biochemistry Principle of Separation techniques Methods Based on Polarity ( ). Biochemistry and Molecular Biology Partition Chromatography Ion Exchange Chromatography Gel Filtration Chromatography Affinity Chromatography 1. How Does Chromatography Work? Chromatography is a method for separating the components of a mixture by differential adsorption between a stationary phase and a mobile (moving) phase Mobile Phase ( ). Liquid Liquid Liquid Solid Partition Adsorption Stationary Phase Chromatography Chromatography ( ). 2. Principles of Separation Techniques AB Molecular Physical property Separation Technique Characteristic Polarity Volatility Gas-liquid Chromatography Solubility Liquid-liquid Chromatography Adsorptivity Liquid-solid Chromatography Ionic Charge Ion-exchange Chromatography Electrophoresis Gel permeation Size (mass) Diffusion Chromatography Dialysis Shape Sedimentation Ultracentrifugation Liquid binding Affi
25 Detection Methods UV – Ultraviolet light--- most popular – Lamp – Grating/Lens - Wave length 190-350 nm – FlowCell – PhotoDiode - Differential Light Output RI – Refractive Index – Universal analyte detector
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