Transcription of SEPARATION TECHNIQUES - uspbpep.com
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SEPARATION TECHNIQUES - uspbpep.com
Chromatographic SEPARATION techniquesEUROPEAN PHARMACOPOEIA , by passing the pressurised solvent through apulse-damping device. Tubing and connections are capableof withstanding the pressures developed by the controlled systems are capable of accuratelydelivering a mobile phase in either constant or varyingconditions, according to a defined programme. In the case ofgradient elution, pumping systems which deliver solvent(s)from several reservoirs are available and solvent mixing canbe achieved on either the low or high-pressure side of thepump(s).InjectorsInjection may be carried out directly at the head of thecolumn using a phasesStationary phases are contained in columns which havebeen described in the chapters onLiquid chromatography( ) (packed columns) andGas chromatography( )(capillary columns). A capillary column has a maximuminternal diameter ( ) of 100 phasesUsually the mobile phase is carbon-dioxide which may containa polar modifier such as methanol, 2-propanol or composition, pressure (density), temperature and flowrateoftheprescribedmobilephasemayeit herbeconstantthroughout the whole chromatographic procedure (isocratic,isodense, isothermic elution) or may vary according toa defined programme (gradient elution of the modifier,pressure ())
EUROPEAN PHARMACOPOEIA 6.0 2.2.46. Chromatographic separation techniques Mass distribution ratio The mass distribution ratio (Dm) (also known as the capacityfactor k’ or retention factor k) is defined as: KC = equilibrium distribution coefficient (also known as distribution constant),
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