SHORT COMMUNICATION Tomas Rodriguez-Riano …

Sex Plant Reprod (2000) 12:241 244 Springer-Verlag 2000. S H O R T C O M M U N I C AT I O N. Tomas Rodriguez-Riano Amots Dafni A new procedure to asses pollen viability Received: 7 May 1999 / Revision accepted: 25 October 1999. Abstract We tested pollen viability of eight species us- that can germinate from that which can not? In this ing four vital dyes, a new peroxidase test together with work, we try to answer the two questions by comparing three other established methods (MTT, Baker's and three common vital dyes and a new peroxidase test with X-Gal), to determine their potential to differentiate fresh in vitro germination of fresh and killed pollen in order to pollen from pollen heated for 2 h and 24 h at 80 C test their reliability, and thus determine their potential ef- (killed pollen ) and compared the results with in vitro ficacy as indicators of pollen viability .

Abstract We tested pollen viability of eight species us-ing four vital dyes, a new peroxidase test together with three other established methods (MTT, Baker’s and

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  Pollen, Viability, Pollen viability

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