Transcription of Small RNA and DNA from Plasma - Macherey …
{{id}} {{{paragraph}}}
Example: dental hygienist
Small RNA and DNA from Plasma - Macherey …
Small RNA and DNA. from Plasma User manual NucleoSpin miRNA Plasma December 2017 / Rev. 05. Isolation of miRNA from Plasma Protocol-at-a-glance (Rev. 05). NucleoSpin miRNA Plasma 300 L Plasma or serum*. 90 L MLP. 1 Prepare sample Vortex 5 s RT, 3 min 30 L MPP. Vortex 5 s 2 Precipitate protein RT, 1 min 11,000 x g, 3 min Transfer clear supernatant to 3 Transfer supernatant Collection Tube (2 mL, lid). 4 Adjust binding 400 L isopropanol conditions Vortex 5 s Load sample on NucleoSpin . miRNA Column 5 Bind RNA and DNA. RT, 2 min 11,000 x g, 30 s Optional: 700 L MW2. 1st 11,000 x g, 30 s 6 Optional: 250 L MW2. 2nd 11,000 x g, 2 min Digest DNA. 50 L rDNase in Reaction Buffer for rDNase RT, 15 min 100 L MW1. 1st 11,000 x g, 30 s 700 L MW2. 7 Wash and dry 2nd 11,000 x g, 30 s 250 L MW2. 3rd 11,000 x g, 2 min 30 L RNase-free H2O. 8 Elute RNA RT, 1 min 11,000 x g, 1 min * Larger sample volumes can be processed when buffer volumes of MLP, MPP, and isopropanol are increased proportionally.
MACHEREY-NAGEL – 12/2017, Rev. 05 3 Small RNA and DNA from plasma Table of contents 1 Components 4 1.1 Kit contents 4 1.2 Reagents, consumables, and equipment to be supplied by user 5
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document: