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Specimen Collection, Handling, Transport and Processing

Specimen collection , Handling, Transport and Processing 1 Part 1: Specimen collection Handling and Transport 2 Specimen Quality is Important The results of tests, as they affect patient diagnosis and treatment, are directly related to the quality of the Specimen collected and delivered to the laboratory. 3 Working with Healthcare Providers Laboratories must develop a good working relationship with health care providers collecting patient specimens Laboratories should have a reference manual for providers that includes: Specimen type and volume requirements Specimen collection , labeling, storage and Transport instructions Specimen rejection criteria Laboratories should provide specific feedback to individual healthcare providers regarding problems with the quality of specimens received and provide recommendations for improvement 4 Specimen Types I. Respiratory Sputum (expectorated, induced) Bronchoalveolar lavage (BAL) Bronchial wash/brush Transtracheal aspirate 5 II.

• Labeling of specimen does not match identifiers on requisition form • Insufficient volume • Dried swabs –Swabs in general are not optimal •Provide limited material •Hydrophobicity of mycobacterial cell envelope inhibits transfer to media • Pooled sputum or urine • Sputum left at room temperature for 24 hours 21

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