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Split/Splitless Gas Chromatography Injection

Split/SplitlessGasChromatographyInjectio nThomas G. ChasteenDepartment of ChemistrySam Houston State UniversitySee ~chemistry/primers/ for a PDF version of this diameter and chromatographic film amount of modern capillary gas chromatographic (GC) columnsdictates that the size of injected samples (the mass of analytes inject on-column) is relatively small compared topacked columns. For instance, packed columns can handle tenths of milligrams (100s of micrograms) example, analyte peaks A and B can be successfully separated with masses of 200 g A and 125 g B andstill get baseline separation (the chromatographer s holy grail). With packed columns this is all capillary columns are much more easily overloaded: A and B need to the 20 g and g at a maximumto be baseline separated if they re closely eluting (similar retention times).

analyte can be instantly varied before an injection is made. This is called the split ratio and is a function of the ratio of gas flows through the GC’s injector, all controlled by pressure/gas flows in the injector. Wait, wait it’ll get better. In older instruments this was carried out by manual control of gas valves. By in modern GCs this is

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Split/Splitless Gas Chromatography Injection - Huntsville, TX

www.shsu.edu

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