Transcription of SPRI Based Size Selection - Fred Hutch
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SPRI Based Size Selection - Fred Hutch
B24965 AAOctober 2012 User GuideSPRI select User GuideSPRI Based size SelectionBeckman Coulter, S. Kraemer , CA 92821 User GuidePN B24965AA (October 2012) 2012 Beckman Coulter, Inc. All rights reservedThe Beckman Coulter stylized logo, SPRI and SPRI select are trademarks of Beckman Coulter, Inc. and are registered in the other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective us on the World Wide Web at: in iiiRevision HistoryInitial Issue, 10/2012 SPRI select User Guide version B24965 AAB24965AA ivRevision History B24965AA vSafety NoticeDo not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter for Warning, Caution, Important, and NoteWARNINGThe signal word WARNING is displayed in an orange signal panel and the associated text (in this example the definition of WARNING) is in indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.
Transfer the eluate (size selected sample) to an appropriate storage vessel. B24965AA 1-5 SPRI Based Size Selection Right Side Size Selection ... Add the required volume of SPRIselect, using the calculation below, to the supernatant from Step 4 above. This will bind the fragments in the supernatant to the new SPRI beads.
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