Transcription of Stains for Developing TLC Plates - McMaster University
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Stains for Developing TLC Plates - McMaster University
Stains for Developing TLC Plates Once a TLC has been developed, it is frequently necessary to aid in the visualization of the components of a reaction mixture. This is true primarily because most organic compounds are colorless. Frequently, the organic compounds of interest contain a chromophore which may be visualized by employing either a short or a long wave UV lamp. These lamps may be found as part of a standard organic chemistry research or teaching lab. Typical examples of functional groups which may be visualized through this method are aromatic groups, , -unsaturated carbonyls, and any other compounds containing extensively -conjugated systems. While exposing these TLC Plates to UV light, you will notice that the silica gel will fluoresce, while any organic molecule which absorbs UV light will appear as a dark blue spot.
Stains for Developing TLC Plates Once a TLC has been developed, it is frequently necessary to aid in the visualization of the components of a reaction mixture. This is true ... • Handbook of Thin-Layer Chromatography J. Sherman and B. Fried, Eds., Marcel Dekker, New York, NY, …
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