Transcription of Standard guide to fluorescence: instrument calibration and ...
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NISTIR 7458 Standard guide to Fluorescence - instrument calibration and Validation Paul C. DeRose Biochemical Science Division National Institute of Standards and Technology Gaithersburg, MD 20899-8312 1 NISTIR 7458 Standard guide to Fluorescence - instrument calibration and Validation Paul C. DeRose Biochemical Science Division National Institute of Standards and Technology Gaithersburg, MD 20899-8312 October 2007 DEPARTMENT OF COMMERCE Carlos M. Gutierrez,Secretary TECHNOLOGY ADMINISTRATION Robert C.
Standard Guide to Fluorescence -Instrument Calibration . and Validation . Paul C. DeRose . ... signals are typically observed, e.g., by a reference detector, and alignment is more difficult than for the emission wavelength accuracy determination. Fig. 1: Hg pen lamp spectrum .
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