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NIST Special Publication 1200-24 Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans Version M. E. Johnson A. R. Montoro Bustos S. K. Hanna E. J. Petersen K. E. Murphy L. L. Yu B. C. Nelson M. R. Winchester This publication is available free of charge from: NIST Special Publication 1200-24 Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans Version M. E. Johnson A. R. Montoro Bustos S. K. Hanna E.
engineered nanomaterials, a critical step for accurate quantification in uptake studies. Conventional rinsing procedures, typically comprised of water or buffer rinsing steps followed by centrifugation, have been pr eviously tested and deemed unsuitable for this purpose, even at environmentally relevant exposure concentrations.
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