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NIST Special Publication 1200-24 Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans Version M. E. Johnson A. R. Montoro Bustos S. K. Hanna E. J. Petersen K. E. Murphy L. L. Yu B. C. Nelson M. R. Winchester This publication is available free of charge from: NIST Special Publication 1200-24 Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans Version M. E. Johnson A. R. Montoro Bustos S. K. Hanna E. J. Petersen K. E. Murphy L.
The construction of the sucrose density gradient column within a centrifuge tube from top to bottom: 2 mL of the nanosuspension (D) or 2 mL of the nematode/nanosuspension mixture (E), 2 mL of 100 µmol/L NaCl (light green) + sucrose [4 mL of 20 % (mass density; purple), 3 mL of 40 % (mass density; orange), and 2 mL of 50 %
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