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NIST Special Publication 1200-24 Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans Version M. E. Johnson A. R. Montoro Bustos S. K. Hanna E. J. Petersen K. E. Murphy L. L. Yu B. C. Nelson M. R. Winchester This publication is available free of charge from: NIST Special Publication 1200-24 Sucrose density gradient centrifugation for efficient separation of engineered nanoparticles from a model organism, Caenorhabditis elegans Version M. E. Johnson A. R. Montoro Bustos S. K. Hanna E. J. Petersen K. E. Murphy L. L. Yu B. C. Nelson M.
Representative schematics of (A) mixed-stage nematodes, (B) the nanosuspension containing 30 nm and 60 nm AuNPs at 100 ng mL-1, respectively, and (C) the nematode/nanosuspension mixture. The construction of the sucrose density gradient column within a centrifuge tube from top to bottom: 2 mL of the nanosuspension (D) or 2 mL of the
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