Transcription of Supporting Information - Cornell University
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Supporting Information - Cornell University
Supporting Information Copyright Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, 2014A Fluorimetric Readout Reporting the Kinetics ofNucleotide-Induced Human Ribonucleotide ReductaseOligomerizationYuan Fu,[a]Hong-Yu Lin,[a]Somsinee Wisitpitthaya,[a]William A. Blessing,[a]andYimon Aye*[a, b] Information Fu et al. 1 CONTENTS General Materials and Methods S-2 Supporting Methods Fluorescent dye labeling of His6- and D57N- S-3 Quantitation of labeling efficiency S-4 LC-MS/MS determination of modified sites S-4 Analytical gel filtration S-5 Activity of labeled proteins S-5 Steady-state fluorescence assays S-6 Stopped-flow fluorescence measurements S-7 Derivation of Eq 1 S-8 Derivation of Eq S-9 Su
Supporting Information Fu et al. ! 3 based on the DTNB-coupled spectrophotometric assay(5).Protein concentrations were determined using ε 280nm /M −1cm−1: 119,160 and 45,900 for α or D57N-α, and β (calculated from the amino acid sequence using the ProtParam tool
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