Transcription of Symmetry Columns - Waters
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Symmetry Columns - Waters
[ CARE AND USE MANUAL ]1 CONTENTSI. INTRODUCTIONII. GETTING STARTED a. Column Installation b. Column Equilibration c. Initial Column Efficiency Determination III. COLUMN USE a. Guard Columns b. Sample Preparation c. pH Range d. Solvents e. Pressure f. TemperatureIV. SCALING UP/DOWN ISOCRATIC METHODSV. TROUBLESHOOTINGVI. COLUMN CLEANING, REGENERATING AND STORAGE a. Cleaning and Regenerating b. StorageVII. CONNECTING THE COLUMN TO THE HPLC a. Column Connectors and System Tubing Considerations b. Band Spreading Minimization c. Cartridge Installation d. Measuring System Bandspreading Volume & System Variance e. Measuring System VolumeVIII. ADDITIONAL INFORMATION a. Use of Narrow-Bore ( mm ) Columns b. Impact of Bandspreading Volume on mm Column Performance c.
µ˚≤˝˙˚ˇ˚ ˚˝ˇ ˝˚ 1 CONTENTS I. INTRODUCTION II. GETTING STARTED a. Column Installation b. Column Equilibration c. Initial Column Efficiency Determination
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