Transcription of Symmetry Columns - Waters Corporation
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Symmetry Columns - Waters Corporation
[ CARE AND USE MANUAL ]1 CONTENTSI. INTRODUCTIONII. GETTING STARTED a. Column Installation b. Column Equilibration c. Initial Column Efficiency Determination III. COLUMN USE a. Guard Columns b. Sample Preparation c. pH Range d. Solvents e. Pressure f. TemperatureIV. SCALING UP/DOWN ISOCRATIC METHODSV. TROUBLESHOOTINGVI. COLUMN CLEANING, REGENERATING AND STORAGE a. Cleaning and Regenerating b. StorageVII. CONNECTING THE COLUMN TO THE HPLC a. Column Connectors and System Tubing Considerations b. Band Spreading Minimization c. Cartridge Installation d. Measuring System Bandspreading Volume & System Variance e. Measuring System VolumeVIII. ADDITIONAL INFORMATION a. Use of Narrow-Bore ( mm ) Columns b. Impact of Bandspreading Volume on mm Column Performance c. Non-Optimized vs. Optimized LC-MS/MS System: System Modification Recommendations d.
Waters Small Particle Size (3.5 µm) ... Flush column with 100% organic mobile phase (methanol or acetonitrile) by setting the pump flow rate to 0.1 mL/min ... One option to avoid this is to use Oasis™ solid-phase extraction cartridges/columns or Sep-Pak™ cartridges of the
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