Transcription of TaKaRa Ex Taq®
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TaKaRa Ex Taq®
PCR test :Good performance of DNA amplification by PCR was confirmed using DNA as the template (amplified fragment: 20 kb).Good performance of DNA amplification of a -globin gene fragment by PCR was also confirmed using human genomic DNA as the template (amplified fragment: kb).General reaction mixture for PCR (total 50 l) : TaKaRa Ex Taq (5 units/ l) l 10X Ex Taq Buffer 5 l dNTP Mixture ( mM each) 4 l Template < 500 ng Primer 1 - M (final conc.) Primer 2 - M (final conc.) Sterilized distilled water up to 50 lExample of PCR conditions : When amplifying a 1 kb DNA fragment98 C 10 C 30 C 1 cycles or98 C 10 C 1 cycles(Note) Denaturation conditions vary depending on the thermal cycler and tubes used for PCR. We recommend denaturing for 5 - 10 sec. at 98 C or 20 - 30 sec. at 94 C.< Cool Start Method >The Cool Start Method provides more accurate amplification and mini-mizes amplification of nonspecific bands.
PCR test : Good performance of DNA amplification by PCR was confirmed using λDNA as the template (amplified fragment: 20 kb). Good performance of DNA amplification of a β-globin gene fragment
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