Transcription of The Bacterial Reverse Mutation Test
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The Bacterial Reverse Mutation Test
CHAPTER 4 The Bacterial Reverse Mutation TestAnnie Hamel1, Marise Roy1and Ray Proudlock21 Department of Genetic Toxicology, Charles River Laboratories, Montreal, ULC, Canada2 Boone, North Carolina, USAC hapter Introduction History Fundamentals Equipment Consumables Reagents and Recipes Ampicillin 2 g/disc Biotin mg/mL Crystal Violet 5 g/disc Glucose g/mL 1M: Glucose-6-Phosphate : 500 M Histidine, 500 M Biotin, 500 M Tryptophan Solution Histidine 5 mg/mL KMg MGA Plates Minimal Glucose Master (MGM, MGMA and MGMAT) Plates NADP M Nutrient Agar Plates Nutrient Broth Phosphate Buffer M pH Positive Control and Diagnostic Mutagen Solutions S9 Fraction S9 Mix Tetracycline 1 g/disc Top Agar Incomplete: TAI Top Agar Complete: TAC Tryptophan 5 mg/mL VB Salts 503: Vogel-Bonner Salts Suggested Phases in Development of the Test The Bacterial Strains Genotypes of Routinely Used Strains Obtaining the Tester Strains 9579 Genetic Toxicology : 2016 Elsevier Inc.
into the animal (often intraperitoneally in mouse), recovered, and plated a few hours after treatment of the animal with the chemical [12]. However, recovery was variable and the system was laborious and not very sensitive. Malling [13] used a mouse liver microsomal fraction to convert dimethylnitrosamine to a bacterial mutagen. Bruce Ames ...
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