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The BLAST Sequence Analysis Tool

The BLAST Sequence Analysis tool [Chapter 16]Tom MaddenSummaryThe comparison of nucleotide or protein sequences from the same or different organisms is a verypowerful tool in molecular biology. By finding similarities between sequences, scientists can inferthe function of newly sequenced genes, predict new members of gene families, and exploreevolutionary relationships. Now that whole genomes are being sequenced, Sequence similaritysearching can be used to predict the location and function of protein-coding and transcription-regulation regions in genomic Local Alignment Search tool ( BLAST ) (1, 2) is the tool most frequently used for calculatingsequence similarity.

sequence, separated by vertical bars (Appendix 1); (b) a brief textual description of the sequence, the definition. This usually includes information on the organism from which the sequence was derived, the type of sequence (e.g., mRNA or DNA), and some information about function or phenotype.

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