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The BLAST Sequence Analysis Tool

The BLAST Sequence Analysis tool [Chapter 16]Tom MaddenSummaryThe comparison of nucleotide or protein sequences from the same or different organisms is a verypowerful tool in molecular biology. By finding similarities between sequences, scientists can inferthe function of newly sequenced genes, predict new members of gene families, and exploreevolutionary relationships. Now that whole genomes are being sequenced, Sequence similaritysearching can be used to predict the location and function of protein-coding and transcription-regulation regions in genomic Local Alignment Search tool ( BLAST ) (1, 2) is the tool most frequently used for calculatingsequence similarity. BLAST comes in variations for use with different query sequences againstdifferent databases.

The query sequence is represented by the numbered red bar at the top of the figure. Database hits are shown aligned to the query, below the red bar. Of the aligned sequences, the most similar are shown closest to the query. In this case, there are three high-scoring database matches that align to most of the query sequence.

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