Transcription of The Emerging Generation of Chromatography …
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The Emerging Generation of Chromatography …
24 BioProcess International Oc tO b e r 2008 SupplementVACCINES PURIFICATIONThe Emerging Generation of Chromatography Tools for Virus Purification by Pete Gagnon Chromatography media and methods have evolved continuously since their introduction a half century ago. Traditional methods use columns packed with porous particles. They still dominate Chromatography applications in the field of virus purification, but the past 20 years have witnessed the ascendance of alternative supports, namely membranes and monoliths. These newer media exploit the familiar surface chemistries ion exchange, hydrophobic interaction, and affinity but they use unique architectures that offer compelling performance features. Th E Ar C h I T E C Tu rE o f Ch r o mA To g rA p h y mE d I AA monolith can be defined as a continuous stationary phase cast as a homogeneous column in a single piece (1 3).
24 BioProcess International Oc t O b e r 2008 Supplement VACCINES PURIFICATION The Emerging Generation of Chromatography Tools for …
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