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THE ROLE OF RELATIVE RESPONSE FACTOR IN RELATED …

, (2011), 919-943 ISSN: 0974-1496 CODEN: RJCABP STUDIES OF RRF BY HPLC Chakravarthy et al. THE ROLE OF RELATIVE RESPONSE FACTOR IN RELATED SUBSTANCES METHOD development BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) V Chakravarthy*, G. Kishore Babu, R. Lakshmana Dasu, P. Prathyusha and G. Aparna Kiran analytical research & development , Natco pharma limited, Hyderabad. Pin Code: 500034, Andhra Pradesh, India. *E-mail: ABSTRACT A study was conducted on RELATIVE RESPONSE FACTOR by changing the High Performance Liquid Chromatography (HPLC) chromatographic method conditions like different HPLC columns, Flow rate, pH, Temperature, Buffer concentration, Detector wavelength, different Detectors (Ultraviolet & Photo Diode Array Detectors) and different Solvent grades and observed the variations in established RRF.

SUBSTANCES METHOD DEVELOPMENT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) V V.Kalyana Chakravarthy*, G. Kishore Babu, R. Lakshmana Dasu, P. Prathyusha and G. Aparna Kiran Analytical Research & Development, Natco pharma limited, Hyderabad. Pin Code: 500034, Andhra Pradesh, India. *E-mail: Kalyan224@rediffmail.com ABSTRACT

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  Development, Research, Methods, Analytical, Method development, Analytical research

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