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Thin Layer Chromatography (TLC) Guide

thin Layer Chromatography (TLC) Guide Overview: thin Layer Chromatography (TLC) is an extremely useful technique for monitoring reactions. It is also used to determine the proper solvent system for performing separations using column Chromatography . TLC uses a stationary phase, usually alumina or silica, that is highly polar (standard) or non-polar (reverse phase), and a mobile phase, some solvent whose polarity you will choose. In , and in most lab applications, you will use standard phase silica plates. You will apply your reaction mixture in solution to the plate then "run" the plate by allowing a solvent (or combination of solvents) to move up the plate by capillary action.

8.3 – Thin Layer Chromatography (TLC) Guide Overview: Thin Layer Chromatography (TLC) is an extremely useful technique for monitoring

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