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Tips for practical HPLC analysis

tips for practical hplc analysis Separation Know-how Shimadzu LC World Talk Special Issue Volume 2C190-E094 2 3 ContentsPage1. Preparation of Mobile Phases42. Differences between Acetonitrile and Methanol in Reversed Phase Chromatography63. Preparing Mobile phases - Solvent Mixing Ratio -84. Preparing Buffer Solutions95. pKa and Dissociation Equilibrium106. Water Grade117. Gradient Baseline for Acetonitrile Containing TFA128. Ion-Pair Chromatography - Choosing between Alkyl Sulfonate and Perchloric Acid -149. Measuring Accurately with Electronic Balances1610.

HPLC-type acetonitrile has the lowest absorbance (particularly for short wavelengths). Using an organic solvent with lower absorbance as the mobile phase results in less noise in UV detection, and so HPLC-type acetonitrile is suitable for high-sensitivity analysis in the UV short-wavelength range. Also, using HPLC-type acetonitrile

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